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cervical epithelial cell line end1  (ATCC)


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    ATCC cervical epithelial cell line end1
    Cell migration and invasion assays as well as cellular affinity of 68Ga-DOTA-TMTP1. A and B. Representative micrographs and quantification of wound healing assay for C33A and Hela tumor cells at 0 h and 24 h (Bar =100 μm). C and D. Transwell invasion assay for C33A and Hela tumor cells (Bar =20 μm). Quantification of migration and invasion assays were calculated by three independent experiments. E. Cell uptake assay of 68Ga-DOTA-TMTP1 in <t>End1,</t> C33A and Hela cells. F. Cell efflux assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. Data are expressed as mean ± SD. G and H. Cell uptake blocking experiments in Hela and C-33A cells. **P < 0.01, ***P < 0.001.
    Cervical Epithelial Cell Line End1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cervical+epithelial+cell+line+end1+e6e7/End1%2FE6E7/pmc11087289-170-2-21
    Average 95 stars, based on 191 article reviews
    cervical epithelial cell line end1 - by Bioz Stars, 2026-10
    95/100 stars

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    1) Product Images from "68 Ga-labeled TMTP1 radiotracer for PET imaging of cervical cancer"

    Article Title: 68 Ga-labeled TMTP1 radiotracer for PET imaging of cervical cancer

    Journal: American Journal of Nuclear Medicine and Molecular Imaging

    doi: 10.62347/NFDH6303

    Cell migration and invasion assays as well as cellular affinity of 68Ga-DOTA-TMTP1. A and B. Representative micrographs and quantification of wound healing assay for C33A and Hela tumor cells at 0 h and 24 h (Bar =100 μm). C and D. Transwell invasion assay for C33A and Hela tumor cells (Bar =20 μm). Quantification of migration and invasion assays were calculated by three independent experiments. E. Cell uptake assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. F. Cell efflux assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. Data are expressed as mean ± SD. G and H. Cell uptake blocking experiments in Hela and C-33A cells. **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: Cell migration and invasion assays as well as cellular affinity of 68Ga-DOTA-TMTP1. A and B. Representative micrographs and quantification of wound healing assay for C33A and Hela tumor cells at 0 h and 24 h (Bar =100 μm). C and D. Transwell invasion assay for C33A and Hela tumor cells (Bar =20 μm). Quantification of migration and invasion assays were calculated by three independent experiments. E. Cell uptake assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. F. Cell efflux assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. Data are expressed as mean ± SD. G and H. Cell uptake blocking experiments in Hela and C-33A cells. **P < 0.01, ***P < 0.001.

    Techniques Used: Migration, Wound Healing Assay, Transwell Invasion Assay, Blocking Assay

    Related Articles

    Cell Culture:

    Article Title: Cervical Cancer Cells-Secreted Exosomal microRNA-221-3p Promotes Invasion, Migration and Angiogenesis of Microvascular Endothelial Cells in Cervical Cancer by Down-Regulating MAPK10 Expression
    Article Snippet: The sections were further incubated with the secondary goat anti-rabbit antibody against immunoglobulin G (IgG) (1: 100, ab6758, Abcam Inc., Cambridge, MA, UK) at 37oC for 20 min. Next, the sections were subjected to incubation with horse radish peroxidase (HRP)-labeled streptavidin ovalbumin solution, visualized using diaminobenzidine (DAB), and then counterstained by hematoxylin (PT001, Shanghai Bogoo Biotechnology Co., Ltd., Shanghai, China) for 1 min. Further, ammonia water was added to the sections for attaining a corresponding blue color, dehydrated using gradient ethanol, cleared by xylene, and finally mounted using a neutral gum and observed under a microscope. .. CC cell lines [Caski ((ATCC ® CRM-CRL-1550), Hela (ATCC ® CCL-2), SiHa (ATCC ® HTB-35) and SW756 (ATCC ® CRL-10302)] and the normal cervical epithelial cell line End1/E6E7 (ATCC ® CRL-2615) [all from American Type Culture Collection (ATCC)] were cultured together in a 37oC incubator supplemented with 5% CO 2 . ..

    Article Title: <p>Cervical Cancer Cells-Secreted Exosomal microRNA-221-3p Promotes Invasion, Migration and Angiogenesis of Microvascular Endothelial Cells in Cervical Cancer by Down-Regulating MAPK10 Expression</p>
    Article Snippet: The sections were further incubated with the secondary goat anti-rabbit antibody against immunoglobulin G (IgG) (1: 100, ab6758, Abcam Inc., Cambridge, MA, UK) at 37oC for 20 min. Next, the sections were subjected to incubation with horse radish peroxidase (HRP)-labeled streptavidin ovalbumin solution, visualized using diaminobenzidine (DAB), and then counterstained by hematoxylin (PT001, Shanghai Bogoo Biotechnology Co., Ltd., Shanghai, China) for 1 min. Further, ammonia water was added to the sections for attaining a corresponding blue color, dehydrated using gradient ethanol, cleared by xylene, and finally mounted using a neutral gum and observed under a microscope. .. Cell Culture and Transfection CC cell lines [Caski ((ATCC® CRM-CRL-1550), Hela (ATCC® CCL-2), SiHa (ATCC® HTB-35) and SW756 (ATCC® CRL-10302)] and the normal cervical epithelial cell line End1/E6E7 (ATCC® CRL-2615) [all from American Type Culture Collection (ATCC)] were cultured together in a 37oC incubator supplemented with 5% CO2. ..

    Transfection:

    Article Title: <p>Cervical Cancer Cells-Secreted Exosomal microRNA-221-3p Promotes Invasion, Migration and Angiogenesis of Microvascular Endothelial Cells in Cervical Cancer by Down-Regulating MAPK10 Expression</p>
    Article Snippet: The sections were further incubated with the secondary goat anti-rabbit antibody against immunoglobulin G (IgG) (1: 100, ab6758, Abcam Inc., Cambridge, MA, UK) at 37oC for 20 min. Next, the sections were subjected to incubation with horse radish peroxidase (HRP)-labeled streptavidin ovalbumin solution, visualized using diaminobenzidine (DAB), and then counterstained by hematoxylin (PT001, Shanghai Bogoo Biotechnology Co., Ltd., Shanghai, China) for 1 min. Further, ammonia water was added to the sections for attaining a corresponding blue color, dehydrated using gradient ethanol, cleared by xylene, and finally mounted using a neutral gum and observed under a microscope. .. Cell Culture and Transfection CC cell lines [Caski ((ATCC® CRM-CRL-1550), Hela (ATCC® CCL-2), SiHa (ATCC® HTB-35) and SW756 (ATCC® CRL-10302)] and the normal cervical epithelial cell line End1/E6E7 (ATCC® CRL-2615) [all from American Type Culture Collection (ATCC)] were cultured together in a 37oC incubator supplemented with 5% CO2. ..



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    Cell migration and invasion assays as well as cellular affinity of 68Ga-DOTA-TMTP1. A and B. Representative micrographs and quantification of wound healing assay for C33A and Hela tumor cells at 0 h and 24 h (Bar =100 μm). C and D. Transwell invasion assay for C33A and Hela tumor cells (Bar =20 μm). Quantification of migration and invasion assays were calculated by three independent experiments. E. Cell uptake assay of 68Ga-DOTA-TMTP1 in <t>End1,</t> C33A and Hela cells. F. Cell efflux assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. Data are expressed as mean ± SD. G and H. Cell uptake blocking experiments in Hela and C-33A cells. **P < 0.01, ***P < 0.001.
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    Cell migration and invasion assays as well as cellular affinity of 68Ga-DOTA-TMTP1. A and B. Representative micrographs and quantification of wound healing assay for C33A and Hela tumor cells at 0 h and 24 h (Bar =100 μm). C and D. Transwell invasion assay for C33A and Hela tumor cells (Bar =20 μm). Quantification of migration and invasion assays were calculated by three independent experiments. E. Cell uptake assay of 68Ga-DOTA-TMTP1 in <t>End1,</t> C33A and Hela cells. F. Cell efflux assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. Data are expressed as mean ± SD. G and H. Cell uptake blocking experiments in Hela and C-33A cells. **P < 0.01, ***P < 0.001.
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    Image Search Results


    Cell migration and invasion assays as well as cellular affinity of 68Ga-DOTA-TMTP1. A and B. Representative micrographs and quantification of wound healing assay for C33A and Hela tumor cells at 0 h and 24 h (Bar =100 μm). C and D. Transwell invasion assay for C33A and Hela tumor cells (Bar =20 μm). Quantification of migration and invasion assays were calculated by three independent experiments. E. Cell uptake assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. F. Cell efflux assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. Data are expressed as mean ± SD. G and H. Cell uptake blocking experiments in Hela and C-33A cells. **P < 0.01, ***P < 0.001.

    Journal: American Journal of Nuclear Medicine and Molecular Imaging

    Article Title: 68 Ga-labeled TMTP1 radiotracer for PET imaging of cervical cancer

    doi: 10.62347/NFDH6303

    Figure Lengend Snippet: Cell migration and invasion assays as well as cellular affinity of 68Ga-DOTA-TMTP1. A and B. Representative micrographs and quantification of wound healing assay for C33A and Hela tumor cells at 0 h and 24 h (Bar =100 μm). C and D. Transwell invasion assay for C33A and Hela tumor cells (Bar =20 μm). Quantification of migration and invasion assays were calculated by three independent experiments. E. Cell uptake assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. F. Cell efflux assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. Data are expressed as mean ± SD. G and H. Cell uptake blocking experiments in Hela and C-33A cells. **P < 0.01, ***P < 0.001.

    Article Snippet: The human cervical epithelial cell line End1 and the human cervical cancer cell lines C-33A and Hela were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Migration, Wound Healing Assay, Transwell Invasion Assay, Blocking Assay